| - | No. 1 Singles |
| R1 | Amber Achenbach (DSUWT) def. Aubrey Erickson (DELVAL-W) 6-3, 6-1 |
| R1 | Amanda Phillips (ALVWT) def. Melanie Haas (LVCW) 6-3, 6-1 |
| R1 | Nicole DelRosso (FDUW) def. Jenna Skove (STE) 6-1, 6-1 |
| R1 | Megan Carter (HOOD) def. Renay Charley (MVILLEWT) 6-3, 6-3 |
| R1 | Kaitlyn Russell (MESSW) def. Zoe Foglia (AUW) 6-1, 6-1 |
| R1 | Emma Paugh (EUWT) def. Allison Mattucci (MIWT) 6-0, 6-2 |
| R1 | Dana Saliyev (WUWT) def. Hannah Summerson (LYCOW) 6-3, 6-3 |
| QF | Emily Fernandez (KCWT18) def. Amber Achenbach (DSUWT) 6-0, 6-0 |
| QF | Nicole DelRosso (FDUW) def. Amanda Phillips (ALVWT) 6-7 (4-7), 6-0, 10-6 |
| QF | Kaitlyn Russell (MESSW) def. Megan Carter (HOOD) 6-4, 5-7, 10-6 |
| QF | Dana Saliyev (WUWT) def. Emma Paugh (EUWT) 6-2, 6-2 |
| SF | Emily Fernandez (KCWT18) def. Nicole DelRosso (FDUW) 6-0, 6-0 |
| SF | Dana Saliyev (WUWT) def. Kaitlyn Russell (MESSW) 6-3, 7-5 |
| F | Emily Fernandez (KCWT18) def. Dana Saliyev (WUWT) 6-2, 6-2 |
| - | No. 2 Singles |
| R1 | Kirsten Kosa (DSUWT) def. Natalie Coffee (KCWT18) 6-2, 6-0 |
| R1 | Lizza Gonzalez (MVILLEWT) def. Allie Vaccaro (DELVAL-W) 6-3, 6-0 |
| R1 | Christine Kenny (FDUW) def. Kirstyn Davidson (STE) 6-1, 6-2 |
| R1 | Ariana Chuprinsky (ALVWT) def. Brittany Lenze (LYCOW) 6-0, 6-0 |
| R1 | Dasha Yakhnis (WUWT) def. Skylar Wright (HOOD) 6-2, 6-0 |
| R1 | Lauren Good (EUWT) def. Rachel Duong (LVCW) 6-2, 6-0 |
| R1 | Emily Wichert (MESSW) def. Hannah Bonk (MIWT) 6-2, 6-3 |
| QF | Tess Weber (AUW) def. Kirsten Kosa (DSUWT) 7-6 (7-5), 3-6, 10-7 |
| QF | Lizza Gonzalez (MVILLEWT) def. Christine Kenny (FDUW) 6-0, 6-0 |
| QF | Dasha Yakhnis (WUWT) def. Ariana Chuprinsky (ALVWT) 6-0, 0-6, 10-7 |
| QF | Emily Wichert (MESSW) def. Lauren Good (EUWT) 6-4, 6-1 |
| SF | Lizza Gonzalez (MVILLEWT) def. Tess Weber (AUW) 6-1, 6-3 |
| SF | Dasha Yakhnis (WUWT) def. Emily Wichert (MESSW) 6-3, 6-3 |
| F | Lizza Gonzalez (MVILLEWT) def. Dasha Yakhnis (WUWT) 6-2, 6-2 |
| - | No. 3 Singles |
| R1 | Chloe Dennerlein (DELVAL-W) def. Samantha Altman (MVILLEWT) walkover |
| R1 | Alex Rodier (DSUWT) def. Martina Gale (KCWT18) 6-4, 4-6, 10-4 |
| R1 | Beka Adams (MESSW) def. Emily Thek (ALVWT) 6-3, 7-5 |
| R1 | Megan Waskiewicz (STE) def. K. Parroccini (LYCOW) 6-2, 6-1 |
| R1 | Julia Paraboschi (AUW) def. Cassidy Breen (MIWT) 6-1, 3-6, 10-6 |
| R1 | Meridyth Sanders (LVCW) def. Jennifer Slick (HOOD) 6-4, 6-1 |
| QF | F. Varela-Seri (WUWT) def. Chloe Dennerlein (DELVAL-W) 6-1, 6-0 |
| QF | Beka Adams (MESSW) def. Alex Rodier (DSUWT) 7-5, 7-6 (7-3) |
| QF | Julia Paraboschi (AUW) def. Megan Waskiewicz (STE) 6-1, 6-2 |
| QF | Allyson Wolf (FDUW) def. Meridyth Sanders (LVCW) 6-2, 6-1 |
| SF | F. Varela-Seri (WUWT) def. Beka Adams (MESSW) 6-1, 6-1 |
| SF | Julia Paraboschi (AUW) def. Allyson Wolf (FDUW) 6-0, 6-3 |
| F | F. Varela-Seri (WUWT) def. Julia Paraboschi (AUW) 6-1, 6-0 |
| - | No. 4 Singles |
| R1 | Emily Wilczek (LVCW) def. Erin Volov (DELVAL-W) 6-2, 6-3 |
| R1 | Alyssa Santarelli (KCWT18) def. Abbey Bloom (HOOD) 6-1, 6-1 |
| R1 | Erika Slowicki (MIWT) def. Sarah Summerson (MESSW) 6-1, 6-2 |
| R1 | Sarah Chan (DSUWT) def. Allyson Dugan (LYCOW) 6-2, 6-1 |
| R1 | Emily Cruz-Pineda (AUW) def. Izzy Lovejoy (MVILLEWT) 6-2, 7-5 |
| R1 | Laura Covely (ALVWT) def. Kelsi Mentzer (STE) 6-1, 6-2 |
| QF | Erin Volutza (WUWT) def. Emily Wilczek (LVCW) 6-0, 6-0 |
| QF | Erika Slowicki (MIWT) def. Alyssa Santarelli (KCWT18) 4-6, 6-0, 10-8 |
| QF | Emily Cruz-Pineda (AUW) def. Sarah Chan (DSUWT) 6-4, 6-3 |
| QF | Kira Bradley (FDUW) def. Laura Covely (ALVWT) 7-5, 6-2 |
| SF | Erin Volutza (WUWT) def. Erika Slowicki (MIWT) 6-2, 6-1 |
| SF | Kira Bradley (FDUW) def. Emily Cruz-Pineda (AUW) 6-3, 6-3 |
| F | Erin Volutza (WUWT) def. Kira Bradley (FDUW) 6-2, 6-0 |
| - | No. 5 Singles |
| R1 | Rachel Marcroft (MESSW) def. Kayla Rembish (MIWT) 7-6 (7-4), 6-1 |
| R1 | Diana Rodriguez (KCWT18) def. Iqra Rafiq (HOOD) 6-0, 6-0 |
| R1 | Cassandra Bean (ALVWT) def. Natalie Clarke (DSUWT) 6-1, 4-6, 10-2 |
| R1 | Elizabeth Drda (LVCW) def. Wiliana Lundy (LYCOW) 6-1, 6-3 |
| R1 | Miller Huertgen (DELVAL-W) def. Kelsey Ulzheimer (MVILLEWT) 6-3, 6-3 |
| R1 | Nina Bretz (STE) def. Casey Boldt (AUW) 2-6, 6-3, 10-6 |
| QF | Rachel Thomas (WUWT) def. Rachel Marcroft (MESSW) 6-4, 6-2 |
| QF | Cassandra Bean (ALVWT) def. Diana Rodriguez (KCWT18) 6-4, 6-3 |
| QF | Elizabeth Drda (LVCW) def. Miller Huertgen (DELVAL-W) 6-0, 6-1 |
| QF | Mia Capozzoli (FDUW) def. Nina Bretz (STE) 7-5, 6-2 |
| SF | Rachel Thomas (WUWT) def. Cassandra Bean (ALVWT) 6-0, 6-0 |
| SF | Mia Capozzoli (FDUW) def. Elizabeth Drda (LVCW) 5-7, 6-1, 10-8 |
| F | Rachel Thomas (WUWT) def. Mia Capozzoli (FDUW) 7-5, 6-0 |
| - | No. 6 Singles |
| R1 | Brooke Dorsey (FDUW) def. Nicki Fox (DELVAL-W) 6-0, 6-0 |
| R1 | Laryssa Badulak (AUW) def. Robin Schwarzmann (MESSW) 6-4, 6-2 |
| R1 | Nicole Joers (MIWT) def. Lauren Fitzgerald (MVILLEWT) 6-0, 6-1 |
| R1 | Bailey Greco (DSUWT) def. Alexis Zalutko (KCWT18) 6-7 (3-7), 6-3, 10-8 |
| QF | Sarah Malone (WUWT) def. Brooke Dorsey (FDUW) 6-1, 6-0 |
| QF | Laryssa Badulak (AUW) def. Esha Kashmiri (STE) 6-1, 6-7 (5-7), 10-5 |
| QF | Leah Wingert (LVCW) def. Nicole Joers (MIWT) 7-5, 6-2 |
| QF | Rachel Rineer (ALVWT) def. Bailey Greco (DSUWT) 2-6, 6-3, 10-5 |
| SF | Sarah Malone (WUWT) def. Laryssa Badulak (AUW) 6-2, 6-3 |
| SF | Rachel Rineer (ALVWT) def. Leah Wingert (LVCW) 6-1, 6-0 |
| F | Sarah Malone (WUWT) def. Rachel Rineer (ALVWT) 6-3, 6-0 |
| - | No. 7 Singles |
| R1 | Sarah Rosario (AUW) def. Nicole Pangiochi (MIWT) 6-4, 6-1 |
| R1 | Hannah Murray (MESSW) def. Jasmin Huertas (MVILLEWT) 6-2, 6-1 |
| R1 | Alexa Cieri (WUWT) def. Jackie Nesbeth (STE) 6-2, 6-2 |
| QF | Sarah Rosario (AUW) def. Thea Seasock (KCWT18) 7-6 (8-6), 6-1 |
| QF | Hannah Murray (MESSW) def. Elizabeth Danovich (ALVWT) 6-4, 6-3 |
| QF | Alexa Cieri (WUWT) def. Kelsey Kazlauskas (DSUWT) 6-2, 6-0 |
| QF | Kaitlyn Welch (LVCW) def. Erin Weller (FDUW) 6-1, 7-6 (7-5) |
| SF | Hannah Murray (MESSW) def. Sarah Rosario (AUW) 7-5, 6-2 |
| SF | Alexa Cieri (WUWT) def. Kaitlyn Welch (LVCW) 6-1, 6-1 |
| F | Alexa Cieri (WUWT) def. Hannah Murray (MESSW) 6-0, 6-0 |
